In addition to clinical services, RGCC also works in research and development, to identify, produce and test potential drugs. We combine molecular biology and cytogenetic techniques – including microarrays, array comparative genomic hybridisation, ChIP, and fluorescence in situ hybridisation – to identify potential genes and proteins that can be targets for drug treatments.
If the drug target is located either outside the cell or in the cell membrane, RGCC’s cellular department is able to produce fully human monoclonal antibodies, and test the efficacy of the antibody with flow cytometry and viability assays.
For intracellular drug targets the RGCC combinatorial chemistry laboratory is able to design and validate in silico different candidate molecules.
The next steps include biochemical assays, which ensure the affinity of the product with the appropriate protein or gene. For small molecular weight targets, we use techniques like IC50 and the Lipinski rule of five before we proceed to further cellular assays. These include viability assays, as well as gene and protein analysis, to validate the products. Every step is controlled with different platforms to ensure the efficacy and reliability of each technique, and increase the chance of the product being a new candidate drug.